Functional characterization of a ClC transporter by solid-supported membrane electrophysiology.

ABSTRACT: EcClC, a prokaryotic member of the ClC family of chloride channels and transporters, works as coupled H(+)/Cl(-) exchanger. With a known structure and the possibility of investigating its behavior with different biochemical and biophysical techniques, the protein has become an important model system for the family. Although many aspects of its function have been previously characterized, it was difficult to measure transport on the same sample under different environmental conditions. To overcome this experimental limitation, we have studied EcClC by solid-supported membrane electrophysiology. The large transport-related transient currents and a simple way of relating transport rates to the measured signal have allowed a thorough investigation of ion selectivity, inhibition, and the depend

SUBMITTER: Garcia-Celma J 

PROVIDER: S-EPMC3607819 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature