Intra-platform comparison of 25-mer and 60-mer oligonucleotide Nimblegen DNA microarrays.

ABSTRACT:

Background

We performed a Nimblegen intra-platform microarray comparison by assessing two categories of flax target probes (short 25-mers oligonucleotides and long 60-mers oligonucleotides) in identical conditions of target production, design, labelling, hybridization, image analyses, and data filtering. We compared technical parameters of array hybridizations, precision and accuracy as well as specific gene expression profiles.

Results

Comparison of the hybridization quality, precision and accuracy of expression measurements, as well as an interpretation of differential gene expression in flax tissues were performed. Both array types yielded reproducible, accurate and comparable data that are coherent for expression measurements and identification of differentially expresse

SUBMITTER: Fenart S 

PROVIDER: S-EPMC3608165 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature