Characterization of cytotoxic compound from mangrove derived fungi Irpex hydnoides VB4.

ABSTRACT:

Objective

To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi.

Methods

In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers ITS 1 and ITS4 and sequence was determined using automated sequencers. Blast search sequence similarity was found against the existing non redundant nucleotide sequence database thus, identified as Aspergilus flavus, Hyporcaea lixii, Aspergillus niger, Eutorium amstelodami, Irpex hydnoides and Neurospora crassa. Among the seven isolates, one fungi Irpex hydnoides was selected for further studies. The fungi were grown in sabouraud broth for five days and filtrate were separated and subjected to ethyl acetate for further studies.

Results

Nearly half (49.25%

SUBMITTER: Bhimba BV 

PROVIDER: S-EPMC3609188 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature