Anticancer property of sediment actinomycetes against MCF-7 and MDA-MB-231 cell lines.

ABSTRACT:

Objective

To investigate the anticancer property of marine sediment actinomycetes against two different breast cancer cell lines.

Methods

In vitro anticancer activity was carried out against breast (MCF-7 and MDA-MB-231) cancer cell lines. Partial sequences of the 16s rRNA gene, phylogenetic tree construction, multiple sequence analysis and secondary structure analysis were also carried out with the actinomycetes isolates.

Results

Of the selected five actinomycete isolates, ACT01 and ACT02 showed the IC50 value with (10.13±0.92) and (22.34±5.82) µg/mL concentrations, respectively for MCF-7 cell line at 48 h, but ACT01 showed the minimum (18.54±2.49 µg/mL) level of IC50 value with MDA-MB-231 cell line. Further, the 16s rRNA partial sequences of ACT01, ACT02, ACT03, ACT

SUBMITTER: Ravikumar S 

PROVIDER: S-EPMC3609251 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature