Single-cell measurements of IgE-mediated FcεRI signaling using an integrated microfluidic platform.

ABSTRACT: Heterogeneity in responses of cells to a stimulus, such as a pathogen or allergen, can potentially play an important role in deciding the fate of the responding cell population and the overall systemic response. Measuring heterogeneous responses requires tools capable of interrogating individual cells. Cell signaling studies commonly do not have single-cell resolution because of the limitations of techniques used such as Westerns, ELISAs, mass spectrometry, and DNA microarrays. Microfluidics devices are increasingly being used to overcome these limitations. Here, we report on a microfluidic platform for cell signaling analysis that combines two orthogonal single-cell measurement technologies: on-chip flow cytometry and optical imaging. The device seamlessly integrates cell culture, stimula

SUBMITTER: Liu Y 

PROVIDER: S-EPMC3609784 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature