De novo assembly and characterization of transcriptome using Illumina paired-end sequencing and identification of CesA gene in ramie (Boehmeria nivea L. Gaud).

ABSTRACT:

Background

Ramie fiber, extracted from vegetative organ stem bast, is one of the most important natural fibers. Understanding the molecular mechanisms of the vegetative growth of the ramie and the formation and development of bast fiber is essential for improving the yield and quality of the ramie fiber. However, only 418 expressed tag sequences (ESTs) of ramie deposited in public databases are far from sufficient to understand the molecular mechanisms. Thus, high-throughput transcriptome sequencing is essential to generate enormous ramie transcript sequences for the purpose of gene discovery, especially genes such as the cellulose synthase (CesA) gene.

Results

Using Illumina paired-end sequencing, about 53 million sequencing reads were generated. De novo assembly yielded 43

SUBMITTER: Liu T 

PROVIDER: S-EPMC3610122 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature