Predictive value of the SLC22A18 protein expression in glioblastoma patients receiving temozolomide therapy.

ABSTRACT:

Background

Our previous study showed that SLC22A18 downregulation and promoter methylation were associated with the development and progression of glioma and the elevated expression of SLC22A18 was found to increase the sensitivity of glioma U251 cells to the anticancer drug 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU). In this study, we investigated the predictive value of SLC22A18 promoter methylation and protein expression in glioblastoma multiforme (GBM) patients receiving temozolomide (TMZ) therapy.

Patients and methods

SLC22A18 promoter methylation and protein expression were examined by methylation-specific polymerase chain reaction (MSP) and Western blotting respectively, then we compared SLC22A18 promoter methylation and protein expression in tumor cell explants in r

SUBMITTER: Chu SH 

PROVIDER: S-EPMC3610152 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature