CD27 expression discriminates porcine T helper cells with functionally distinct properties.

ABSTRACT: Differentiation of porcine T helper cells is still poorly investigated, partly due to a lack of monoclonal antibodies (mAbs) specific for molecules involved in this process. Recently, we identified a mAb specific for porcine CD27 and showed that CD27 is expressed by all naïve CD8?- T helper cells but divides CD8?+ T helper cells into a CD27+ and a CD27- subset. In the present study, detailed phenotypical and functional analyses of these T-helper cell subpopulations were performed. Naïve CD8?-CD27+ T helper cells predominantly resided in various lymph nodes, whereas higher proportions of CD8?+CD27+ and CD8?+CD27- T helper cells were found in blood, spleen and liver. Both CD8?+CD27+ and CD8?+CD27- T helper cells were capable of producing IFN-? upon in vitro polyclonal stimulation and antigen-specific restimulation. Experiments with sorted CD8?-CD27+, CD8?+CD27+ and CD8?+CD27- T-helper cell subsets following polyclonal stimulation revealed the lowest proliferative response but the highest ability for IFN-? and TNF-? production in the CD8?+CD27- subset. Therefore, these cells resembled terminally differentiated effector memory cells as described in human. This was supported by analyses of CCR7 and CD62L expression. CD8?+CD27- T helper cells were mostly CCR7- and had considerably reduced CD62L mRNA levels. In contrast, expression of both homing-receptors was increased on CD8?+CD27+ T helper cells, which also had a proliferation rate similar to naïve CD8?-CD27+ T helper cells and showed intermediate levels of cytokine production. Therefore, similar to human, CD8?+CD27+ T helper cells displayed a phenotype and functional properties of central memory cells.

SUBMITTER: Reutner K 

PROVIDER: S-EPMC3610194 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature