Dataset Information
The use of DNA from archival dried blood spots with the Infinium HumanMethylation450 array.
Ontology highlight
ABSTRACT: Background
Dried blood (Guthrie card) spots provide an efficient way to collect and store blood specimens. DNA from this source has been utilised for a number of molecular analyses including genome-wide association studies, but only few studies have tested the feasibility of using it for epigenetic applications, particularly at a genome-wide level.Results
In this study, we demonstrate the successful use of DNA isolated from archived dried blood spots for the Infinium HumanMethylation450 Beadchip, along with DNA from matched frozen buffy coats. We obtained high quality and reproducible genome-wide DNA methylation profiles using both sample types. We also report high correlations (r > 0.9907) between DNA obtained from matched dried blood spots and frozen buffy coats, sufficient to distinguish between unrelated individuals.Conclusions
We, thus, demonstrate that DNA from archived dried blood spots is suitable for genome-wide DNA methylation profiling.
SUBMITTER: Joo JE
PROVIDER: S-EPMC3610215 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Publications
The use of DNA from archival dried blood spots with the Infinium HumanMethylation450 array.
BMC biotechnology 20130315
<h4>Background</h4>Dried blood (Guthrie card) spots provide an efficient way to collect and store blood specimens. DNA from this source has been utilised for a number of molecular analyses including genome-wide association studies, but only few studies have tested the feasibility of using it for epigenetic applications, particularly at a genome-wide level.<h4>Results</h4>In this study, we demonstrate the successful use of DNA isolated from archived dried blood spots for the Infinium HumanMethyla ...[more]