Extracellular matrix-associated gene expression in adult sensory neuron populations cultured on a laminin substrate.

ABSTRACT:

Background

In our previous investigations of the role of the extracellular matrix (ECM) in promoting neurite growth we have observed that a permissive laminin (LN) substrate stimulates differential growth responses in subpopulations of mature dorsal root ganglion (DRG) neurons. DRG neurons expressing Trk and p75 receptors grow neurites on a LN substrate in the absence of neurotrophins, while isolectin B4-binding neurons (IB4+) do not display significant growth under the same conditions. We set out to determine whether there was an expression signature of the LN-induced neurite growth phenotype. Using a lectin binding protocol IB4+ neurons were isolated from dissociated DRG neurons, creating two groups - IB4+ and IB4-. A small-scale microarray approach was employed to screen the exp

SUBMITTER: Fudge NJ 

PROVIDER: S-EPMC3610289 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature