Searching the H. pylori; serology & PCR in children with adenoid hypertrophy and rhino sinusitis: a cross sectional study, Tehran, Iran.

ABSTRACT:

Objective

Searching the H. pylori -DNAs in adenoid and IgA, IgG antibodies in serum of children.

Methods

A cross- sectional study had done upon 53 children (Mean age 8 ±1.9 years) with adenoid surgery in ENT and Pediatric Department of Rasul Akram Hospital (2008-2010). Of 53 cases with adenoid surgery, 40 cases had rhino sinusitis (in sinus CT scan). The resected adenoid tissues (n=40) centrifuged and homogenized and its DNAs extracted and searched for H. pylori- DNAs by qualitative PCR. Serum H. pylori antibodies (IgG &IgA ELISA) calculated quantitatively. Chi square values (p < 0.05) calculated for all categorical variables. The agreement between serologic test and PCR was assessed by the calculation of kappa statistic.

Results

Positive PCR for H. pylori detected in

SUBMITTER: Farhadi M 

PROVIDER: S-EPMC3610311 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature