Gene regulation by CcpA and catabolite repression explored by RNA-Seq in Streptococcus mutans.

ABSTRACT: A bacterial transcriptome of the primary etiological agent of human dental caries, Streptococcus mutans, is described here using deep RNA sequencing. Differential expression profiles of the transcriptome in the context of carbohydrate source, and of the presence or absence of the catabolite control protein CcpA, revealed good agreement with previously-published DNA microarrays. In addition, RNA-seq considerably expanded the repertoire of DNA sequences that showed statistically-significant changes in expression as a function of the presence of CcpA and growth carbohydrate. Novel mRNAs and small RNAs were identified, some of which were differentially expressed in conditions tested in this study, suggesting that the function of the S. mutans CcpA protein and the influence of carbohydrate sour

SUBMITTER: Zeng L 

PROVIDER: S-EPMC3610829 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature