High resolution methylome analysis reveals widespread functional hypomethylation during adult human erythropoiesis.

ABSTRACT: Differentiation of hematopoietic stem cells to red cells requires coordinated expression of numerous erythroid genes and is characterized by nuclear condensation and extrusion during terminal development. To understand the regulatory mechanisms governing these widespread phenotypic changes, we conducted a high resolution methylomic and transcriptomic analysis of six major stages of human erythroid differentiation. We observed widespread epigenetic differences between early and late stages of erythropoiesis with progressive loss of methylation being the dominant change during differentiation. Gene bodies, intergenic regions, and CpG shores were preferentially demethylated during erythropoiesis. Epigenetic changes at transcription factor binding sites correlated significantly with changes in

SUBMITTER: Yu Y 

PROVIDER: S-EPMC3610956 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature