Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily.

ABSTRACT: The number of available protein sequences has increased exponentially with the advent of high-throughput genomic sequencing, creating a significant challenge for functional annotation. Here, we describe a large-scale study on assigning function to unknown members of the trans-polyprenyl transferase (E-PTS) subgroup in the isoprenoid synthase superfamily, which provides substrates for the biosynthesis of the more than 55,000 isoprenoid metabolites. Although the mechanism for determining the product chain length for these enzymes is known, there is no simple relationship between function and primary sequence, so that assigning function is challenging. We addressed this challenge through large-scale bioinformatics analysis of >5,000 putative polyprenyl transferases; experimental characterizat

SUBMITTER: Wallrapp FH 

PROVIDER: S-EPMC3612614 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature