A transgenic insect cell line engineered to produce CMP-sialic acid and sialylated glycoproteins.

ABSTRACT: We have previously engineered transgenic insect cell lines to express mammalian glycosyltransferases and showed that these cells can sialylate N-glycoproteins, despite the fact that they have little intracellular sialic acid and no detectable CMP-sialic acid. In the accompanying study, we presented evidence that these cell lines can salvage sialic acids for de novo glycoprotein sialylation from extracellular sialoglycoproteins, such as fetuin, found in fetal bovine serum. This finding led us to create a new transgenic insect cell line designed to synthesize its own sialic acid and CMP-sialic acid. SfSWT-1 cells, which encode five mammalian glycosyltransferases, were transformed with two additional mammalian genes that encode sialic acid synthase and CMP-sialic acid synthetase. The resultin

SUBMITTER: Aumiller JJ 

PROVIDER: S-EPMC3612900 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature