Unknown

Dataset Information

0

Gan-Lu-Yin Inhibits Proliferation and Migration of Murine WEHI-3 Leukemia Cells and Tumor Growth in BALB/C Allograft Tumor Model.


ABSTRACT: The aim of this study was to explore the antitumor effect of Gan-Lu-Yin (GLY), a traditional Chinese herbal formula, on leukemia. Ethanolic extract of GLY was applied to evaluate its regulatory mechanisms in proliferation, migration, and differentiation of WEHI-3 leukemic cells as well as antitumor effect on BALB/c mice model. The results showed that GLY markedly reduced cell proliferation and migration with induced differentiation of WEHI-3 cells. The expression level of phosphorylated FAK, Akt, ERK1/2, and Rb was decreased p21 expression while level was increased in WEHI-3 treated with GLY. The results of cell cycle analysis revealed that GLY treatment could markedly induce G1 phase arrest and decrease cell population in S phase. Moreover, experimental results demonstrated that GLY decreased the protein expression and enzyme activity of MMP-2 and MMP-9. GLY treatment also reduced WEHI-3 leukemic infiltration in liver and spleen and tumor growth in animal model. Accordingly, GLY demonstrated an inhibitory effect on tumor growth with a regulatory mechanism partially through inhibiting FAK, Akt, and ERK expression in WEHI-3 cells. GLY may provide a promising antileukemic approach in the clinical application.

SUBMITTER: Liu FC 

PROVIDER: S-EPMC3613066 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

altmetric image

Publications

Gan-Lu-Yin Inhibits Proliferation and Migration of Murine WEHI-3 Leukemia Cells and Tumor Growth in BALB/C Allograft Tumor Model.

Liu Fon-Chang FC   Pan Chun-Hsu CH   Lai Ming-Tsung MT   Chang Shu-Jen SJ   Chung Jing-Gung JG   Wu Chieh-Hsi CH  

Evidence-based complementary and alternative medicine : eCAM 20130317


The aim of this study was to explore the antitumor effect of Gan-Lu-Yin (GLY), a traditional Chinese herbal formula, on leukemia. Ethanolic extract of GLY was applied to evaluate its regulatory mechanisms in proliferation, migration, and differentiation of WEHI-3 leukemic cells as well as antitumor effect on BALB/c mice model. The results showed that GLY markedly reduced cell proliferation and migration with induced differentiation of WEHI-3 cells. The expression level of phosphorylated FAK, Akt  ...[more]

Similar Datasets

| S-EPMC7926381 | biostudies-literature
2012-12-27 | E-GEOD-43145 | biostudies-arrayexpress
2012-12-27 | GSE43145 | GEO
| S-EPMC3360742 | biostudies-literature
| S-EPMC6280296 | biostudies-literature
2021-06-15 | GSE178087 | GEO
| S-EPMC6375810 | biostudies-literature
| S-EPMC4199906 | biostudies-literature
| S-EPMC5634572 | biostudies-literature
| S-EPMC5954458 | biostudies-literature