High-resolution mapping, characterization, and optimization of autonomously replicating sequences in yeast.

ABSTRACT: DNA replication origins are necessary for the duplication of genomes. In addition, plasmid-based expression systems require DNA replication origins to maintain plasmids efficiently. The yeast autonomously replicating sequence (ARS) assay has been a valuable tool in dissecting replication origin structure and function. However, the dearth of information on origins in diverse yeasts limits the availability of efficient replication origin modules to only a handful of species and restricts our understanding of origin function and evolution. To enable rapid study of origins, we have developed a sequencing-based suite of methods for comprehensively mapping and characterizing ARSs within a yeast genome. Our approach finely maps genomic inserts capable of supporting plasmid replication and uses ma

SUBMITTER: Liachko I 

PROVIDER: S-EPMC3613586 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature