Apoptotic microtubules delimit an active caspase free area in the cellular cortex during the execution phase of apoptosis.

ABSTRACT: Apoptotic microtubule network (AMN) is organized during apoptosis, forming a cortical structure beneath plasma membrane, which has an important role in preserving cell morphology and plasma membrane permeability. The aim of this study was to examine the role of AMN in maintaining plasma membrane integrity during the execution phase of apoptosis. We demonstrated in camptothecin-induced apoptosis in H460 cells that AMN delimits an active caspase free area beneath plasma membrane that permits the preservation of cellular cortex and transmembrane proteins. AMN depolymerization in apoptotic cells by a short exposure to colchicine allowed active caspases to reach the cellular cortex and cleave many key proteins involved in plasma membrane structural support, cell adhesion and ionic homeostasis.

SUBMITTER: Oropesa-Avila M 

PROVIDER: S-EPMC3613836 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature