Effect of cargo size and shape on the transport efficiency of the bacterial Tat translocase.

ABSTRACT: The Tat machinery translocates fully-folded and oligomeric substrates. The passage of large, bulky cargos across an ion-tight membrane suggests the need to match pore and cargo size, and therefore that Tat transport efficiency may depend on both cargo size and shape. A series of cargos of different sizes and shapes were generated using the natural Tat substrate pre-SufI as a base. Four (of 17) cargos transported with significant (>20% of wild-type) efficiencies. These results indicate that cargo size and shape significantly influence Tat transportability.

SUBMITTER: Whitaker N 

PROVIDER: S-EPMC3613985 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature