Short-term culture of ovarian cortex pieces to assess the cryopreservation outcome in wild felids for genome conservation.

ABSTRACT:

Background

Cryopreservation of ovarian tissue has the potential to preserve female germ cells of endangered mammals. In the present study, a freezing protocol successfully used for human tissue, was adapted for preserving ovarian tissue of domestic and non-domestic felids. Ovaries from non-domestic felid species were obtained from seven freshly euthanized and two recently deceased wild felids kept in different European Zoos. In addition, ovaries from domestic cats were obtained after ovariectomy from local veterinary clinics for methological adaptations.Ovarian cortex was dissected and uniform sized pieces of 2 mm diameter were obtained. Using a slow freezing protocol (-0.3°C per min) in 1.5 mol/L ethylene glycol, 0.1 mol/L sucrose, the pieces were cultured for up to 14 days both b

SUBMITTER: Wiedemann C 

PROVIDER: S-EPMC3614466 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature