Diversity of the var gene family of Indonesian Plasmodium falciparum isolates.

ABSTRACT:

Background

The large polymorphic protein PfEMP1 is encoded by the var gene family. PfEMP1 has been shown to play an important role as cytoadherence ligand on the surface of infected erythrocytes and thereby contributes to the distinct pathogenesis of malaria. The study explored the diversity of the DBL1α and DBL2β-C2 domains of the protein from Indonesian Plasmodium falciparum field isolates.

Methods

Samples of patients with severe and uncomplicated malaria from two different malaria-endemic areas in Indonesia were collected and DNA directly extracted. Dried blood on filter paper was prepared for RNA extraction. PCR amplicons were either cloned and subsequently sequenced or directly sequenced for analysis on nucleotide and amino acid level. Recently published as well as self

SUBMITTER: Sulistyaningsih E 

PROVIDER: S-EPMC3614516 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature