Rapid detection of SNP (c.309T>G) in the MDM2 gene by the Duplex SmartAmp method.

ABSTRACT:

Background

Genetic polymorphisms in the human MDM2 gene are suggested to be a tumor susceptibility marker and a prognostic factor for cancer. It has been reported that a single nucleotide polymorphism (SNP) c.309T>G in the MDM2 gene attenuates the tumor suppressor activity of p53 and accelerates tumor formation in humans.

Methodology

In this study, to detect the SNP c.309T>G in the MDM2 gene, we have developed a new SNP detection method, named "Duplex SmartAmp," which enabled us to simultaneously detect both 309T and 309G alleles in one tube. To develop this new method, we introduced new primers i.e., nBP and oBPs, as well as two different fluorescent dyes that separately detect those genetic polymorphisms.

Results and conclusions

By the Duplex SmartAmp method, the ge

SUBMITTER: Enokida Y 

PROVIDER: S-EPMC3614994 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature