Analyses of the effects of all ubiquitin point mutants on yeast growth rate.

ABSTRACT: The amino acid sequence of a protein governs its function. We used bulk competition and focused deep sequencing to investigate the effects of all ubiquitin point mutants on yeast growth rate. Many aspects of ubiquitin function have been carefully studied, which enabled interpretation of our growth analyses in light of a rich structural, biophysical and biochemical knowledge base. In one highly sensitive cluster on the surface of ubiquitin, almost every amino acid substitution caused growth defects. In contrast, the opposite face tolerated virtually all possible substitutions. Surface locations between these two faces exhibited intermediate mutational tolerance. The sensitive face corresponds to the known interface for many binding partners. Across all surface positions, we observe a strong

SUBMITTER: Roscoe BP 

PROVIDER: S-EPMC3615125 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature