Phosphatidylinositol-3-phosphate clearance plays a key role in autophagosome completion.

ABSTRACT:

Background

The biogenesis of autophagosomes, the hallmark of autophagy, depends on the function of the autophagy-related (Atg) proteins and the generation of phosphatidylinositol-3-phosphate (PtdIns3P) at the phagophore assembly site (PAS), the location where autophagosomes arise. The current model is that PtdIns3P is involved primarily in the recruitment of Atg proteins to the PAS and that once an autophagosome is complete, the Atg machinery is released from its surface back into the cytoplasm and reused for the formation of new vesicles.

Results

We have identified a PtdIns3P phosphatase, Ymr1, that is essential for the normal progression of both bulk and selective types of autophagy. This protein is recruited to the PAS at an early stage of formation of this structure thro

SUBMITTER: Cebollero E 

PROVIDER: S-EPMC3615650 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature