Pregnancy prediction in single embryo transfer cycles after ICSI using QPCR: validation in oocytes from the same cohort.

ABSTRACT: Cumulus cell (CC) gene expression is being explored as an additional method to morphological scoring to choose the embryo with the highest chance to pregnancy. In 47 ICSI patients with single embryo transfer (SET), from which individual CC samples had been stored, 12 genes using QPCR were retrospectively analyzed. The CC samples were at the same occasion also used to validate a previously obtained pregnancy prediction model comprising three genes (ephrin-B2 (EFNB2), calcium/calmodulin-dependent protein kinase ID, stanniocalcin 1). Latter validation yielded a correct pregnant/non-pregnant classification in 72% of the samples. Subsequently, 9 new genes were analyzed on the same samples and new prediction models were built. Out of the 12 genes analyzed a combination of the best predictive gen

SUBMITTER: Wathlet S 

PROVIDER: S-EPMC3616108 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature