Temozolomide-mediated radiosensitization of human glioma cells in a zebrafish embryonic system.

ABSTRACT: The zebrafish (Danio rerio) is a popular vertebrate model for biomedical research. The rapid development, transparency, and experimental accessibility of the embryo offer opportunities for assessing the developmental effects of anticancer treatment strategies. We therefore systematically investigated parameters for growing U251 human glioma cells expressing red fluorescent protein (U251-RFP) in zebrafish embryos. Factors optimized include injection volume, number of cells injected, anatomic site of injection, age of the embryo at the time of injection, and postinjection incubation temperature. After injection into the embryos, the U251-RFP cells proliferated and the resultant tumors, and even individual cells, could be visualized in real-time via fluorescence microscopy without the need fo

SUBMITTER: Geiger GA 

PROVIDER: S-EPMC3616326 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature