Global remodeling of the vascular stem cell niche in bone marrow of diabetic patients: implication of the microRNA-155/FOXO3a signaling pathway.

ABSTRACT:

Rationale

The impact of diabetes mellitus on bone marrow (BM) structure is incompletely understood.

Objective

Investigate the effect of type-2 diabetes mellitus (T2DM) on BM microvascular and hematopoietic cell composition in patients without vascular complications.

Methods and results

Bone samples were obtained from T2DM patients and nondiabetic controls (C) during hip replacement surgery and from T2DM patients undergoing amputation for critical limb ischemia. BM composition was assessed by histomorphometry, immunostaining, and flow cytometry. Expressional studies were performed on CD34(pos) immunosorted BM progenitor cells (PCs). Diabetes mellitus causes a reduction of hematopoietic tissue, fat deposition, and microvascular rarefaction, especially when associated wi

SUBMITTER: Spinetti G 

PROVIDER: S-EPMC3616365 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature