The use of scaffold-free cell sheet technique to refine mesenchymal stromal cell-based therapy for heart failure.

ABSTRACT: Transplantation of bone marrow-derived mesenchymal stromal cells (MSCs) is an emerging treatment for heart failure based on their secretion-mediated "paracrine effects". Feasibility of the scaffoldless cell sheet technique to enhance the outcome of cell transplantation has been reported using other cell types, though the mechanism underpinning the enhancement remains uncertain. We here investigated the role of this innovative technique to amplify the effects of MSC transplantation with a focus on the underlying factors. After coronary artery ligation in rats, syngeneic MSCs were grafted by either epicardial placement of MSC sheets generated using temperature-responsive dishes or intramyocardial (IM) injection. Markedly increased initial retention boosted the presence of donor MSCs persiste

SUBMITTER: Narita T 

PROVIDER: S-EPMC3616531 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature