Study of PcaV from Streptomyces coelicolor yields new insights into ligand-responsive MarR family transcription factors.

ABSTRACT: MarR family proteins constitute a group of >12 000 transcriptional regulators encoded in bacterial and archaeal genomes that control gene expression in metabolism, stress responses, virulence and multi-drug resistance. There is much interest in defining the molecular mechanism by which ligand binding attenuates the DNA-binding activities of these proteins. Here, we describe how PcaV, a MarR family regulator in Streptomyces coelicolor, controls transcription of genes encoding β-ketoadipate pathway enzymes through its interaction with the pathway substrate, protocatechuate. This transcriptional repressor is the only MarR protein known to regulate this essential pathway for aromatic catabolism. In in vitro assays, protocatechuate and other phenolic compounds disrupt the PcaV-DNA complex. We s

SUBMITTER: Davis JR 

PROVIDER: S-EPMC3616709 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature