Sequence-specific inhibition of Dicer measured with a force-based microarray for RNA ligands.

ABSTRACT: Malfunction of protein translation causes many severe diseases, and suitable correction strategies may become the basis of effective therapies. One major regulatory element of protein translation is the nuclease Dicer that cuts double-stranded RNA independently of the sequence into pieces of 19-22 base pairs starting the RNA interference pathway and activating miRNAs. Inhibiting Dicer is not desirable owing to its multifunctional influence on the cell's gene regulation. Blocking specific RNA sequences by small-molecule binding, however, is a promising approach to affect the cell's condition in a controlled manner. A label-free assay for the screening of site-specific interference of small molecules with Dicer activity is thus needed. We used the Molecular Force Assay (MFA), recently develo

SUBMITTER: Limmer K 

PROVIDER: S-EPMC3616731 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature