A qRT-PCR assay for the expression of all Mal d 1 isoallergen genes.

ABSTRACT:

Background

A considerable number of individuals suffer from oral allergy syndrome (OAS) to apple, resulting in the avoidance of apple consumption. Apple cultivars differ greatly in their allergenic properties, but knowledge of the causes for such differences is incomplete. Mal d 1 is considered the major apple allergen. For Mal d 1, a wide range of isoallergens and variants exist, and they are encoded by a large gene family. To identify the specific proteins/genes that are potentially involved in the allergy, we developed a PCR assay to monitor the expression of each individual Mal d 1 gene. Gene-specific primer pairs were designed for the exploitation of sequence differences among Mal d 1 genes. The specificity of these primers was validated using both in silico and in vitro techn

SUBMITTER: Pagliarani G 

PROVIDER: S-EPMC3616815 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature