Enhanced transgene expression in rice following selection controlled by weak promoters.

ABSTRACT:

Background

Techniques that enable high levels of transgene expression in plants are attractive for the commercial production of plant-made recombinant pharmaceutical proteins or other gene transfer related strategies. The conventional way to increase the yield of desired transgenic products is to use strong promoters to control the expression of the transgene. Although many such promoters have been identified and characterized, the increase obtainable from a single promoter is ultimately limited to a certain extent.

Results

In this study, we report a method to magnify the effect of a single promoter by using a weak promoter-based selection system in transgenic rice. tCUP1, a fragment derived from the tobacco cryptic promoter (tCUP), was tested for its activity in rice by fus

SUBMITTER: Zhou J 

PROVIDER: S-EPMC3617001 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature