Combinatorial pooling enables selective sequencing of the barley gene space.

ABSTRACT: For the vast majority of species - including many economically or ecologically important organisms, progress in biological research is hampered due to the lack of a reference genome sequence. Despite recent advances in sequencing technologies, several factors still limit the availability of such a critical resource. At the same time, many research groups and international consortia have already produced BAC libraries and physical maps and now are in a position to proceed with the development of whole-genome sequences organized around a physical map anchored to a genetic map. We propose a BAC-by-BAC sequencing protocol that combines combinatorial pooling design and second-generation sequencing technology to efficiently approach denovo selective genome sequencing. We show that combinatorial

SUBMITTER: Lonardi S 

PROVIDER: S-EPMC3617026 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature