A pyrosequencing-based metagenomic study of methane-producing microbial community in solid-state biogas reactor.

ABSTRACT:

Background

A solid-state anaerobic digestion method is used to produce biogas from various solid wastes in China but the efficiency of methane production requires constant improvement. The diversity and abundance of relevant microorganisms play important roles in methanogenesis of biomass. The next-generation high-throughput pyrosequencing platform (Roche/454 GS FLX Titanium) provides a powerful tool for the discovery of novel microbes within the biogas-generating microbial communities.

Results

To improve the power of our metagenomic analysis, we first evaluated five different protocols for extracting total DNA from biogas-producing mesophilic solid-state fermentation materials and then chose two high-quality protocols for a full-scale analysis. The characterization of both

SUBMITTER: Li A 

PROVIDER: S-EPMC3618299 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature