Plastome Sequence Determination and Comparative Analysis for Members of the Lolium-Festuca Grass Species Complex.

ABSTRACT: Chloroplast genome sequences are of broad significance in plant biology, due to frequent use in molecular phylogenetics, comparative genomics, population genetics, and genetic modification studies. The present study used a second-generation sequencing approach to determine and assemble the plastid genomes (plastomes) of four representatives from the agriculturally important Lolium-Festuca species complex of pasture grasses (Lolium multiflorum, Festuca pratensis, Festuca altissima, and Festuca ovina). Total cellular DNA was extracted from either roots or leaves, was sequenced, and the output was filtered for plastome-related reads. A comparison between sources revealed fewer plastome-related reads from root-derived template but an increase in incidental bacterium-derived sequences. Plastome

SUBMITTER: Hand ML 

PROVIDER: S-EPMC3618348 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature