Prospects and limits of the flow cytometric seed screen--insights from Potentilla sensu lato (Potentilleae, Rosaceae).

ABSTRACT: The flow cytometric seed screen allows for identification of reproductive modes of seed formation and inference of the ploidy of contributing gametes. However, the lack of a mathematical formalization to infer male/female genomic contributions, and the prerequisite of a binucleate female contribution to the endosperm limits its applicability. We evaluated this assumption combining a DNA-based progeny survey with a comparison of the cytology of reproductive pathways co-occurring within single individuals representing 14 Potentilleae species from six phylogenetic lineages. A numerical framework valid for sexual and pseudogamous taxa was developed, enabling quantification of female and male genomes contributing to embryo and endosperm independent of gametophyte origins, numbers of sperm invol

SUBMITTER: Dobes C 

PROVIDER: S-EPMC3618378 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature