Nod2-induced autocrine interleukin-1 alters signaling by ERK and p38 to differentially regulate secretion of inflammatory cytokines.

ABSTRACT:

Background & aims

Stimulation of nucleotide-binding oligomerization domain-containing (Nod)2 and other pattern recognition receptors (PRR) in human monocyte-derived macrophages induces interleukin (IL)-1, which increases mitogen-activated protein kinase (MAPK) activation and cytokine secretion. Activation of MAPK by PRR has varied effects on inflammatory cytokine secretion. We investigated whether different levels of autocrine IL-1 mediate these varied effects.

Methods

Macrophage responses to PRR ligands were analyzed by enzyme-linked immunosorbent assay and flow cytometry. We overexpressed or reduced MAPK levels (using small inhibitory RNA).

Results

Nod2 and other PRR activated signaling via extracellular signal-related kinase (ERK) and p38 that inhibited inflammator

SUBMITTER: Hedl M 

PROVIDER: S-EPMC3618474 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature