Lubiprostone decreases mouse colonic inner mucus layer thickness and alters intestinal microbiota.

ABSTRACT:

Background

Lubiprostone has been used to treat constipation through its effects to stimulate Cl(-) secretion, resulting in water and electrolyte secretion.

Aim

Potential associated changes in intestinal mucus and the colonizing bacteria (microbiome) have not been studied. As mucus obstructions may play a role in cystic fibrosis, the hypothesis that lubiprostone alters intestinal mucus and the microbiome was investigated.

Methods

Ion transport studies were performed ex vivo. For mucus and microbiome studies, mice were gavaged daily with lubiprostone or vehicle. Mucin from intestinal sections was analyzed in Carnoy's fixed tissues stained with Alcian blue. Microbiome composition was analyzed by 16S rRNA gene-based sequencing.

Results

Lubiprostone stimulated short

SUBMITTER: Musch MW 

PROVIDER: S-EPMC3618493 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature