Subcellular fractionation enhances proteome coverage of pancreatic duct cells.

ABSTRACT:

Objectives

Subcellular fractionation of whole cell lysates offers a means of simplifying protein mixtures, potentially permitting greater depth of proteomic analysis. Here we compare proteins identified from pancreatic duct cells (PaDC) following organelle enrichment to those identified from PaDC whole cell lysates to determine if the additional procedures of subcellular fractionation increase proteome coverage.

Methods

We used differential centrifugation to enrich for nuclear, mitochondrial, membrane, and cytosolic proteins. We then compared - via mass spectrometry-based analysis - the number of proteins identified from these four fractions with four biological replicates of PaDC whole cell lysates.

Results

We identified similar numbers of proteins among all samples

SUBMITTER: Paulo JA 

PROVIDER: S-EPMC3618666 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature