A novel positively charged achiral co-monomer for β-cyclodextrin monolithic stationary phase: improved chiral separation of acidic compounds using capillary electrochromatography coupled to mass spectrometry.

ABSTRACT: The work presented here demonstrates the incorporation of vinylbenzyl trimethylammonium (VBTA) as a novel positively charged achiral co-monomer to a glycidyl methacrylate-beta cyclodextrin (GMA/β-CD) based monolith, providing anion exchange sites with reversed electroosmotic flow (EOF) for capillary electrochromatography (CEC). The monolithic phases, GMA/β-CD-VBTA and GMA/β-CD (without co-monomer) were characterized by scanning electron microscopy, optical microscopy, pressure drop/flow-rate curves and nitrogen adsorption analysis. After optimizing the stationary phase and mobile phase parameters, chiral separations of 41 pairs of structurally diverse anionic chiral analytes were compared individually using the GMA/β-CD-VBTA and GMA/β-CD monolithic columns. The GMA/β-CD-VBTA monolith chira

SUBMITTER: Bragg W 

PROVIDER: S-EPMC3618962 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature