Putative tumour suppressor gene necdin is hypermethylated and mutated in human cancer.

ABSTRACT:

Background

Necdin (NDN) expression is downregulated in telomerase-immortalised normal human urothelial cells. Telomerase-immortalised normal human urothelial cells have no detected genetic alterations. Accordingly, many of the genes whose expression is altered following immortalisation are those for which epigenetic silencing is reported.

Methods

NDN expression was examined in normal tissues and tumour cell lines by quantitative real-time PCR and immunoblotting. Immunohistochemistry was performed on urothelial carcinoma (UC). Urothelial carcinoma and UC cell lines were subject to HumanMethylation27 BeadChip Array-based methylation analyses. Mutation screening was performed. The functional significance of NDN expression was investigated using retroviral-mediated downregulatio

SUBMITTER: De Faveri LE 

PROVIDER: S-EPMC3619261 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature