Boosting the pentose phosphate pathway restores cardiac progenitor cell availability in diabetes.

ABSTRACT:

Aims

Diabetes impinges upon mechanisms of cardiovascular repair. However, the biochemical adaptation of cardiac stem cells to sustained hyperglycaemia remains largely unknown. Here, we investigate the molecular targets of high glucose-induced damage in cardiac progenitor cells (CPCs) from murine and human hearts and attempt safeguarding CPC viability and function through reactivation of the pentose phosphate pathway.

Methods and results

Type-1 diabetes was induced by streptozotocin. CPC abundance was determined by flow cytometry. Proliferating CPCs were identified in situ by immunostaining for the proliferation marker Ki67. Diabetic hearts showed marked reduction in CPC abundance and proliferation when compared with controls. Moreover, Sca-1(pos) CPCs isolated from hearts of

SUBMITTER: Katare R 

PROVIDER: S-EPMC3619276 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature