Changes in fibroblast growth factor-2 and FGF receptors in the frog visual system during optic nerve regeneration.

ABSTRACT: We have previously shown that application of fibroblast growth factor-2 (FGF-2) to cut optic nerve axons enhances retinal ganglion cell (RGC) survival in the adult frog visual system. These actions are mediated via activation of its high affinity receptor FGFR1, enhanced BDNF and TrkB expression, increased CREB phosphorylation, and by promoting MAPK and PKA signaling pathways. The role of endogenous FGF-2 in this system is less well understood. In this study, we determine the distribution of FGF-2 and its receptors in normal animals and in animals at different times after optic nerve cut. Immunohistochemistry and Western blot analysis were conducted using specific antibodies against FGF-2 and its receptors in control retinas and optic tecta, and after one, three, and six weeks post nerve i

SUBMITTER: Duprey-Diaz MV 

PROVIDER: S-EPMC3619718 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature