Performance in omics analyses of blood samples in long-term storage: opportunities for the exploitation of existing biobanks in environmental health research.

ABSTRACT:

Background

The suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown.

Objectives

We evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles.

Methods

We collected fresh blood samples without RNA preservative in heparin, EDTA, or citrate and held them at room temperature for ≤ 24 hr before fractionating them into buffy coat, erythrocytes, and plasma and freezing the fractions at -80oC or in liquid nitrogen. We developed methodology for isolating RNA from the buffy coats and conducted omic

SUBMITTER: Hebels DG 

PROVIDER: S-EPMC3620742 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature