Monitoring intracellular redox changes in ozone-exposed airway epithelial cells.

ABSTRACT:

Background

The toxicity of many xenobiotic compounds is believed to involve oxidative injury to cells. Direct assessment of mechanistic events involved in xenobiotic-induced oxidative stress is not easily achievable. Development of genetically encoded probes designed for monitoring intracellular redox changes represents a methodological advance with potential applications in toxicological studies.

Objective

We tested the utility of redox-sensitive green fluorescent protein (roGFP)-based redox sensors for monitoring real-time intracellular redox changes induced by xenobiotics in toxicological studies.

Methods

roGFP2, a reporter of the glutathione redox potential (E(GSH)), was used to monitor EGSH in cultured human airway epithelial cells (BEAS-2B cells) undergoing expo

SUBMITTER: Gibbs-Flournoy EA 

PROVIDER: S-EPMC3621206 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature