Identification of genes involved in serum tolerance in the clinical strain Cronobacter sakazakii ES5.

ABSTRACT:

Background

Cronobacter spp. are opportunistic pathogens that can cause septicemia and infections of the central nervous system primarily in premature, low-birth weight and/or immune-compromised neonates. Serum resistance is a crucial virulence factor for the development of systemic infections, including bacteremia. It was the aim of the current study to identify genes involved in serum tolerance in a selected Cronobacter sakazakii strain of clinical origin.

Results

Screening of 2749 random transposon knock out mutants of a C. sakazakii ES 5 library for modified serum tolerance (compared to wild type) revealed 10 mutants showing significantly increased/reduced resistance to serum killing. Identification of the affected sites in mutants displaying reduced serum resistance reve

SUBMITTER: Schwizer S 

PROVIDER: S-EPMC3621496 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature