Follicle-stimulating hormone responsiveness in antral follicles from aryl hydrocarbon receptor knockout mice.

ABSTRACT:

Background

Previous studies have demonstrated that pre-pubertal aryl hydrocarbon receptor knockout (AHRKO) mice have slow antral follicle growth and reduced capacity to produce estradiol compared to wild-type (WT) mice. Although previous studies have suggested that this is likely due to a reduced ability of the AHRKO follicles to respond to follicle-stimulating hormone (FSH), this possibility was not directly tested. Thus, the goal of these studies was to test the hypothesis that low FSH responsiveness is responsible for the slow growth and reduced estradiol production observed in pre-pubertal AHRKO versus WT antral follicles.

Methods

Antral follicles from WT and AHRKO mice were cultured with varying amounts of FSH (0-15 IU/mL) for up to 7 days, and subjected to measurements

SUBMITTER: Hernandez-Ochoa I 

PROVIDER: S-EPMC3621516 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature