Genome-scale analyses of butanol tolerance in Saccharomyces cerevisiae reveal an essential role of protein degradation.

ABSTRACT:

Background

n-Butanol and isobutanol produced from biomass-derived sugars are promising renewable transport fuels and solvents. Saccharomyces cerevisiae has been engineered for butanol production, but its high butanol sensitivity poses an upper limit to product titers that can be reached by further pathway engineering. A better understanding of the molecular basis of butanol stress and tolerance of S. cerevisiae is important for achieving improved tolerance.

Results

By combining a screening of the haploid S. cerevisiae knock-out library, gene overexpression, and genome analysis of evolutionary engineered n-butanol-tolerant strains, we established that protein degradation plays an essential role in tolerance. Strains deleted in genes involved in the ubiquitin-proteasome system

SUBMITTER: Gonzalez-Ramos D 

PROVIDER: S-EPMC3621596 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature