Transcript and protein profiling analysis of the Destruxin A-induced response in larvae of Plutella xylostella.

ABSTRACT:

Background

Destruxins (dtxs) are the mycotoxin produced by certain entomopathogenic fungi, such as Metarhizium anisopliae, Aschersonia sp, Alternaria brassicae and Ophiosphaerella herpotrichae. It can affect a wide variety of biological processes in insects, including innate immune, Ca(2+) channel in cells, and apoptosis in a dose-dependent manner. Dtxs have been used as biological control agent for a long time, however, their molecular mechanism of action is still unknown.

Principal findings

In this study, both digital gene expression (DGE) and two-dimensional electrophoresis (2-DE) approaches were adopted to examine the effects of dtx A on Plutella xyllostella (L.) larvae. By using DGE and 2-DE analyses, 1584 genes and 42 protein points were identified as being up- or down

SUBMITTER: Han P 

PROVIDER: S-EPMC3621956 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature