Hydrogen sulphide-induced relaxation of porcine peripheral bronchioles.

ABSTRACT:

Background and purpose

Hydrogen sulphide (H2S) is an endogenous gasotransmitter. Although it has been shown to elicit responses in vascular and other smooth muscle preparations, a role for endogenously produced H2S in mediating airway tone has yet to be demonstrated. Therefore, the aim of this study was to determine whether H2S is produced within the airways and to determine the functional effect on airway tone.

Experimental approach

Small peripheral airways (<5 mm in diameter) from porcine lungs were set up in isolated tissue baths, pre-contracted with the muscarinic agonist carbachol, and then exposed to either the H2S donor sodium hydrosulphide (NaHS), or the precursor L-cysteine. H2S production from L-cysteine or 3-mercaptopyruvate in tissue homogenates was measured by t

SUBMITTER: Rashid S 

PROVIDER: S-EPMC3623060 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature